9/18/2023 0 Comments Arcturus pixcell ii manual![]() ![]() This method utilizes reverse transcription with T7 RNA Polymerase. RNA from three groups of animals (no running, 4 days of running, 30 days of running), consisting of 3 animals each, was isolated independently and amplified separately.ĥng total RNA starting material was amplified using the MessageAmp aRNA Kit (Ambion Inc, Austin, TX). Total RNA was purified from the laser captured tissue using the Absolutely RNA Nanoprep Kit (Stratagene, La Jolla, CA), and RNA yield was assessed using the RiboGreen RNA Quantitation Reagent (Molecular Probes Inc, Eugene, OR). An Arcturus PixCell II machine (Arcturus, Mountain View, CA) was used to isolate 2-3 cell thick bands from both the inner and outer portions of the dentate granule cell layer. 12μm thin cryostat sections were stained with cresyl violet, rapidly dehydrated through xylenes, and stored until use a vacuum dessicator. ![]() Outer granule cell layer from a mouse allowed access to running wheel for 30 daysīrains were quickly removed, flash-frozen in OCT mounting medium in a dry ice-isopentantane slurry, and stored at -80C until use. GEO help: Mouse over screen elements for information.ĭentate gyrus-granule cell layer-30daysRunning, repAT1 ![]()
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